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KMID : 0923620110110060416
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Immune Network
2011 Volume.11 No. 6 p.416 ~ p.419
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Th17 Responses Are Not Induced in Dextran Sodium Sulfate Model of Acute Colitis
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Kim Yoon-Suk
Lee Min-Ho
Ju Ahn-Seung
Rhee Ki-Jong
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Abstract
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Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-? (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
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KEYWORD
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Dextran sodium sulfate, Inflammatory bowel disease, Th17 cell, Colitis
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